Sampling for the E-Tegrity Test

This test requires tissue from the fundus of the uterus collected through a standard endometrial biopsy technique using a Pipelle de Cornier®. It is crucial that the patient have a carefully timed endometrial biopsy for the purpose of testing for ß3 integrin. The specimen MUST be collected 7 to 11 days after the urinary LH surge (determined by the patient using a commercial ovulation detection kit). Once the biopsy is collected the sample should be placed in formalin and submitted to Innovative Reproductive Solutions within 10 days. The presence or absence of ß3 integrin is determined through a special staining technique which is combined with histologic dating of the endometrium to provide a final result. Factors that affect interpretation of the test results are the cycle day of specimen collection, the collection technique, and the length of time the specimen sits in formalin.

Innovative Reproductive Solutions offers the E-tegrity Test which includes Hematoxylin and Eosin stain for histologic dating of the endometrium read by a certified pathologist, as well as the patented test for ß3 integrin.* Both tests must be performed on the same biopsy through the Innovative Reproductive Solutions laboratory. Due to the variability of endometrial tissue dating between different pathologists, only results from Innovative Reproductive Solutions-validated pathologists may be used in the interpretation of the E-tegrity Test. For this reason, formalin-fixed tissue must be submitted directly to the Innovative Reproductive Solutions laboratory. Most results from Innovative Reproductive Solutions are available in approximately one week of receipt of a properly collected specimen. Results will be sent to the ordering physician.

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